How to prepare for Biotechnology and its Applications for CUET-UG ?

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Expected Weightage

The Common University Entrance Test (CUET) for Biotechnology and its Applications has a specific marking pattern and chapter-wise weightage that can be analyzed based on past trends (2022-2024). Below is a detailed breakdown:

1. CUET Biotechnology Marking Pattern (Last 3 Years)

  • Total Questions: 50 (out of which 40 must be attempted)
  • Marks per Correct Answer: +5
  • Marks per Incorrect Answer: -1
  • Unattempted Questions: 0
  • Maximum Marks: 200 (40 × 5)

2. Chapter-wise Weightage (Approximate Trends)

The syllabus is based on NCERT Class 12 Biology (Unit 10: Biotechnology Its Applications). The weightage varies slightly each year, but the following pattern is observed:

Chapter/Topic Weightage (2022-2024) Expected No. of Questions
Principles of Biotechnology (Tools, PCR, Vectors, etc.) 25-30% 8-10
Genetic Engineering (Recombinant DNA Technology) 20-25% 7-9
Biotechnological Applications in Agriculture (GM Crops, Bt Cotton, RNAi, etc.) 15-20% 5-7
Biotechnological Applications in Medicine (Gene Therapy, Vaccines, Insulin, etc.) 15-20% 5-7
Transgenic Animals, Ethical Issues, and Biosafety 10-15% 3-5
Other Topics (Bioinformatics, IPR, Biopatents, etc.) 5-10% 2-4

3. Key Observations (2022-2024)

  • Most Important Chapters:

  • Recombinant DNA Technology (highest weightage)

  • PCR Gene Cloning (frequently asked)

  • GM Crops Bt Toxin (application-based questions)

  • Gene Therapy Insulin Production (medical applications)

  • Least Important Chapters:

  • Ethical Issues Biosafety (fewer questions)

  • Bioinformatics Biopatents (occasionally asked)

  • Question Types:

  • Conceptual (60%) – Definitions, steps in PCR, gene cloning

  • Application-based (30%) – GM crops, gene therapy

  • Diagram-based (10%) – Gel electrophoresis, PCR steps

4. Preparation Strategy

  • Focus Areas:

  • Recombinant DNA Technology (20-25 marks)

  • PCR Cloning Vectors (15-20 marks)

  • Bt Cotton RNAi (10-15 marks)

  • Insulin Production Gene Therapy (10-15 marks)

  • Avoid Neglecting:

  • Transgenic Animals Ethical Issues (5-10 marks)

  • Biopatents Bioinformatics (2-4 marks)

5. Expected Changes (2025 Beyond)

  • More Application-based Questions (especially in medicine agriculture)
  • Increased Focus on Ethical Biosafety Concerns (due to recent biotech advancements)

Conclusion

For CUET Biotechnology , prioritize Recombinant DNA Technology, PCR, GM Crops, and Medical Applications. Solve previous years’ papers (2022-2024) to identify repeated patterns.

Would you like a topic-wise difficulty analysis or recommended books for CUET Biotechnology?

Last edited by a moderator: Jun 16, 2025